StepOne™ Real-Time PCR System
Catalog number: 4376357
Description:
The StepOne™ Real-Time PCR System is a 48-well, low-throughput Real-Time PCR instrument perfect for both first-time and experienced users. The StepOne™ Real-Time PCR System can be setup in a variety of configurations and comes ready to use, out of the box, with intuitive data analysis and instrument control software. Utilizing robust LED based 3-color optical recording, the StepOne™ Real-Time PCR System is designed to deliver precise, quantitative Real-Time PCR results for a variety of genomic research applications.
Features of the The StepOne™ Real-Time PCR System include:
- Advanced software and instrumentation for performing a wide array of genomic assays.
- Sensitive 3-color optical LED recording system
- Intuitive and robust software perfect for both first-time and advanced users
- Simple and flexible instrument set-up and usage
StepOne™ Software
The StepOne™ Software included with the StepOne™ Real-Time PCR System runs on Windows® XP, Window Vista®, and Windows® 7 operating systems and provides instrument control, data collection, and data analysis capabilities. This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.
Rapid and Simple Assay Set-Up
This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOne™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.
Sensitive LED-Based 3-Color Fluorescence Reading
The StepOne™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, and ROX™ dyes. This cost-effective, 3-color, 48-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.
Compatible with Many Genomic Analysis Techniques:
Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOne™ Real-Time PCR System and software. The StepOne™ System supports many Real-Time PCR applications, including the following:
- SNP Genotyping
- Gene Expression Analysis
- MicroRNA Expression
- Translocation Analysis
- Gene Detection
- Viral Load Analysis
The included StepOne™ Software supports a variety of analysis methods, including the following:
- Standard Curve (absolute quantitation)
- Relative Standard Curve
- Comparative Ct (ΔΔCt) (relative quantitation)
- Genotyping and Presence/Absence
- Melt Curve Analysis
- High Resolution Melting (additional software required)
Specifications:
| Calibrated Dye: | FAM™, JOE™, ROX™, SYBR® Green I, VIC® |
|---|---|
| Capacity: | 1 x 48-well plate, 48 x 0.1 ml tubes |
| Detection Method: | Primer-Probe Detection, SYBR |
| Dimensions: | 24.6 cm/9.7 in.(W) x 42.7 cm/16.8 in.(D) x 51.2 cm/20.2 in.(H) |
| Dynamic Range: | Linear Dynamic Range greater than 9 log units (detection) |
| For Use With (Equipment): | StepOne™ |
| Format: | 0.1 ml tubes, 48-well Plate |
| Green Features: | Energy efficient, Fewer resources used and less waste |
| High Throughput Compatibility: | Multiplexing |
| Optics: | 3 emission filters, LED, photodiode |
| Passive Reference Dye: | No ROX, ROX (Pre-mixed), ROX (Separate Tube) |
| Peak Block Ramp Rate: | 4.6°C⁄sec |
| Product Line: | StepOne™ |
| Product Size: | 1 instrument |
| Reaction Speed: | Fast, Standard |
| Reaction Volume Range: | 10-30 µl (Standard curve experiments: 40 µl in standard mode is validated) |
| Run Time: | <2 hrs ⁄ run (Standard Mode), <40 min ⁄ run (Fast Mode) |
| Sample Ramp Rate: | Fast mode: ± 2.2°C ⁄ sec, Standard mode: ± 1.6°C⁄sec |
| Sensitivity: | 1 copy |
| Temperature Accuracy: | 0.25°C (35 to 95°C) of display temperature |
| Temperature Range (Metric): | 4-100°C |
| Temperature Uniformity: | 0.25°C (35 to 95°C) of setpoint/display temperature |
| Thermal Cycling System: | Peltier-Based System |
| Weight: | 23.6 kg (52 lbs.) |